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- W2368562020 abstract "Objective To analyze the application value of Identifiler kit in duo paternity testing.Methods The technique of fluorescent dye labeling multiplex STR-PCR and capillary electrophoresis and DNA sequencer GeneScan.Genetyping of 15 short tandem repeat(STR) were adopted in genetyping a sample of individuals in 276 cases parentage testing by using 3100 Avant.Results Among 276 cases of duo parentage testing,199 cases of determined paternal relation were 72.10% in all cases,and cumulative paternity index(CPI) was 2 000 or more than 2 000.In 183 cases,the relative chance of paternity(RCP) was 99.95% or more than 99.95%.And CPI of 13 case was small than 2 000,3 cases had a step mutation of a gene locus.77 cases were excluded paternity relationship.The percentage was 27.9% in all cases.2 cases had one to two loci incoordinated genetic law in these exclusive cases,then exclusive indexes were more than three items by additional detecting mother′s sample.Conclusion Using Identifiler system of 15 STR loci can successfully analyze duo paternity testing.But worth of watching,when CPI is small than 2 000 or having 1-2 incoordinate gene loci,we must add to determine more loci or sample of mother for improving CPI or exclusive index,and then avoiding error identification conclusion." @default.
- W2368562020 created "2016-06-24" @default.
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- W2368562020 date "2010-01-01" @default.
- W2368562020 modified "2023-09-24" @default.
- W2368562020 title "Usefulness of Identifiler system in 276 cases of duo paternity testing" @default.
- W2368562020 hasPublicationYear "2010" @default.
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