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- W2368565638 abstract "Objective:To isolate protoplasts of leaves in Brassica rapa ‘Tsuda’ and to build the system of transient expression of protein in protoplasts.Methods:The leaves of ‘Tsuda’ turnip were used as materials to isolate protoplast by enzyme digestion.The transient expression vector with reported gene coded green fluorescent protein(GFP) was transferred into protoplast by PEG method,and the expression of GFP in protoplast was examined by laser scanning confocal microscopy.Results:It showed that many protoplasts of leaves were isolated and high efficiency of transformation was obtained.The protein of GFP was examined to express in all over the protoplast.Conclusion:The system of transient expression of target gene in protoplasts of B.rapa ‘Tsuda’ has been established." @default.
- W2368565638 created "2016-06-24" @default.
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- W2368565638 date "2008-01-01" @default.
- W2368565638 modified "2023-09-28" @default.
- W2368565638 title "Protoplast Isolation of Brassica rapa ‘Tsuda’ Turnip and Transient Expression of Green Fluorescent Protein" @default.
- W2368565638 hasPublicationYear "2008" @default.
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