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- W2368585906 abstract "Aim To examine the changes of T lymphocytes in the brain following focal cerebral ischemia with flow cytometry.Methods Focal ischemia was generated by permanent electrocoagulation of middle cerebral artery occlusion under a microscope.Rats were randomly divided into two groups: a sham group and a middle cerebral artery occlusion(MCAO) group.In the sham-operated control,the MCAO was visualized.While 72 hours after MCAO or sham-operation,rats were killed.The brain and blood samples were individually obtained for the examination of T lymphocytes(fluorescence labeled by CD3-APC) by flow cytometry.Results Compared with the sham group,there was a significant increase of CD3+ cells in the infarct cerebral hemisphere 72 h after MCAO(P0.01).However there was no significant change of CD3+ cells in the blood samples 72 h after ischemia compared with the sham group(P0.1).Conclusion The study indicated that flow cytometry could analyze the immune cells of the brain tissue following cerebral ischemia.T lymphocytes in the brain increased significantly after MCAO,which might play a great role in ischemic brain injury or protection or both." @default.
- W2368585906 created "2016-06-24" @default.
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- W2368585906 date "2013-01-01" @default.
- W2368585906 modified "2023-09-24" @default.
- W2368585906 title "The Changes of T Lymphocytes Following Focal Cerebral Ischemia in Rat Examined by Flow Cytometry" @default.
- W2368585906 hasPublicationYear "2013" @default.
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