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- W2368607705 abstract "Plasminogen activator inhibitor 1 (PAI-1) levels are increased in obesity and the metabolic syndrome and heighten the risk of atherothrombotic disease. Since obesity is characterized by adipose tissue macrophage infiltration (JCI 112:1796 and 1821, 2003), we proposed that adipose macrophages might be an important source of circulating PAI-1. We therefore biopsied subcutaneous abdominal (SAT) and omental (OAT) adipose tissue in six nondiabetic elective surgery patients (4 F/2 M, age 33 ± 3 years, BMI 31.9 ± 1.5 kg/m2). Following collagenase digestion and adipocyte filtration, macrophages were separated from other stromal cells by CD14-coated Dynabeads (A80% recovery). Gene expression was quantified by real-time rt-PCR using plasmid standard curves. PAI-1 gene expression was A2-fold higher in SAT vs OAT (whole fat) and 4-fold higher in macrophages and stromal cells than in adipocytes. We previously showed that increasing free fatty acids (FFA) up to levels seen in obesity stimulated PAI-1 gene expression in adipose tissue. Therefore, we hypothesized that FFA have stimulatory effects on PAI-1 expression in adipose tissue macrophages. Subcutaneous abdominal fat was biopsied in 17 obese nondiabetic subjects (5 F/12 M, age 45 ± 4 years, BMI 30 ± 1 kg/m2) after 5-hour euglycemic (5mM) insulin (40 mU/m2.min) clamp studies, with infusion of saline (Sal) or Liposyn 20% (Lip). Increasing FFA to A800 mM for 5 hours induced 5-fold increases in whole adipose tissue PAI-1 gene expression (p" @default.
- W2368607705 created "2016-06-24" @default.
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- W2368607705 date "2006-03-01" @default.
- W2368607705 modified "2023-09-27" @default.
- W2368607705 title "79 ADIPOSE TISSUE MACROPHAGES EXPRESS HIGH LEVELS OF PLASMINOGEN ACTIVATOR INHIBITOR 1 IN HUMANS AND ARE HIGHLY RESPONSIVE TO INCREASED FREE FATTY ACIDS." @default.
- W2368607705 doi "https://doi.org/10.2310/6650.2005.x0015.157" @default.
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