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- W2368644282 abstract "In order to elucidate the timing mechanism of circadian rhythm in silkworm(Bombyx mori),expression patterns of two critical clock genes,Cry1 and Cry2 which encode the cryptochrome of silkworm adult,were investigated under treatment of 4 photoperiods as well as 2 periodic oscillations of temperature.Expression patterns of Cry1 and Cry2 were assayed by Real-time PCR at each developmental stage of silkworm under normal temperature and light condition(25 ℃,LD 12 h∶12 h).It was found that both genes were remarkably up-regulated at the adult stage,while only trace expression could be detected at the embryonic stage and during the 5th instar larval stage.Expression profile of both genes in adult tissues displayed that head tissue had the highest expression level.Meanwhile,the expression level of Cry1 in antennae and Cry2 in flight muscle was also quite high.RT-PCR analyses revealed that oscillatory rhythm existed in the expression of both Cry genes in head,antenna and flight muscle of the adult under different photoperiods,indicating that change of photoperiod could alter expressional oscillatory phase of these two genes.Oscillatory phase of Cry1 expression was altered when the illumination started.In addition,the responses from the head and antenna where central nervous system was located to the flight muscle of peripheral tissue showed a time gradient pattern.However,expression product of Cry2 gene might not be the first substance responding to light zeitgeber.Both Cry genes were sensitive to the periodic oscillation of temperature.Change of oscillating cycle within the circadian period led to an alteration in the oscillatory phase of Cry1 expression and an inversion in the oscillatory wave form of Cry2 expression.It is suggested that both Cry1 and Cry2 are related to signal transformation of temperature in biological clock,but their functions may differ." @default.
- W2368644282 created "2016-06-24" @default.
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- W2368644282 date "2013-01-01" @default.
- W2368644282 modified "2023-09-25" @default.
- W2368644282 title "Effects of Temperature and Light Rhythm on Expression of Clock Genes Cry1 and Cry2 in Bombyx mori Adult" @default.
- W2368644282 hasPublicationYear "2013" @default.
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