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- W2368658214 abstract "Objective To prepare uteroglobin-related protein 1(UGRP1) fusion protein,an unknown secretion protein,and its polyclonal antibody for following function study. Methods The coding region of UGRP1 was amplified from a human embryo lung tissue by RT-PCR and the recombinant prokaryotic expression vector pGEX-UGRP1 was constructed.The vector was transformed into E.coli BL21(DE3) to expresse the glutathione-S-transferase(GST)-UGRP1 fusion protein in the bacteria under induction of isopropyl β-D-1-thiogalactopyranoside(IPTG).After purification,the fusion protein was injected into New Zealand rabbits to prepare polyclonal antibody.Then the antibody was tested by Western Blotting for their sensitivity and specificity.The full sequence of UGRP1 gene was also amplified by RT-PCR and then recombined in the downstream of the green fluorescent protein gene in the EGFP-N2 vector.The recombined vector EGFP-N2-UGRP1 was transfected into COS cells to investigate its expression with laser scanning confocal microscope. Results The recombinant prokaryotic expression vector pGEX-UGRP1 was constructed successfully,then GST-UGRP1 fusion protein and its polyclonal antibody were also generated.Immunohistochemical assay,by using this antibody,demonstrated that UGRP1 had high expression in the epithelial cells of the airways,while it's confirmed that UGRP1 was predominant expression in the cytoplasm. Conclusion Preparation of UGRP1 fusion protein and its polyclonal antibody,together with preliminary study of UGRP1 expression characteristics,may lay foundation to investigating the function of UGRP1 gene in the future." @default.
- W2368658214 created "2016-06-24" @default.
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- W2368658214 date "2007-01-01" @default.
- W2368658214 modified "2023-09-23" @default.
- W2368658214 title "Cloning,expression and polyclonal antibody preparation of UGRP1 and its subcellular localization" @default.
- W2368658214 hasPublicationYear "2007" @default.
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