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- W2368662763 abstract "Objective To study the level of nitric oxide synthase(NOS)in ischemia-reperfusion rat liver.Methods 24 healthy male SD rats were anaesthetized and heparinised.The hepatic vein,portal vein and bile duct were cannulated.The liver was then removed to establish a model of isolated perfusion rat liver(IPRL).The rats were randomly divided into 4 groups(n=6 per group).In GroupⅠ(warm I/R group),the IPRL was formed immediately after removal.The livers of Groups Ⅱto Ⅳwere first stored in cold UW solution for 6,12 and 24 hours,respectively.The livers were then perfused via the portal vein,with freshly prepared Krebs-Bulbring buffer for 30mins.The temperature of organ bath and perfusate was controlled at 38℃.Bile secretion was noted;albumin(ALB) was determined.Histochemistry was performed to measure the activities of hepatic endothelin-1(ET-1),nitric oxide(NO),nitric oxide synthase(NOS),ATPase,superoxide dismutase(SOD),xanthine oxidase(XOD)and malondiaehyde(MDA) separately.Results The activity of NOS was increased significantly as the period of cold storage wad prolonged(P0.05),no distinct changes were found in NO and ET-1,while slight variations were seen in ATPase,SOD,XOD and MDA.Bile was secreted during the ischemia-reperfusin process in all of the models.ALB showed no changes.Conclusion NOS is a sensitive index to reflect the liver ischmia/reperfusion injury." @default.
- W2368662763 created "2016-06-24" @default.
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- W2368662763 date "2006-01-01" @default.
- W2368662763 modified "2023-09-23" @default.
- W2368662763 title "Study on the changes of nitric oxide synthase in ischemia-reperfusion rat liver" @default.
- W2368662763 hasPublicationYear "2006" @default.
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