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- W2368694909 abstract "The leaves cDNA of ‘Dangshansuli’ pear(Pyrus bretschneideri) at different degrees of iron-deficiency were taken as experiment materials.The normal leaves cDNA was taken as CK.The cDNA full length of Fer2 gene was cloned by RACE(rapid-amplification of cDNA ends) method.The results showed that the full length of Fer2 gene was 1 052 bp,and its coding region covered 828 bp and encoded a polypeptide containing 276 amino acids.The physicochemical properties and structures of Fer2 protein were analysed by bioinformatics softwares Protparam,InterProScan and ProtFun 2.2 Server etc.The results showed that Fer2 protein had a higher homology with the protein Fer2 and Fer4 in the leaf of ‘Cuiguan’ pear and was named as PbFer2 gene.The relative molecular mass of PbFer2 protein in the leaf of ‘Dangshansuli’ pear was 3 054.3,and the theoretical isoelectric point was 5.80.The PbFer2 gene expression levels were analyzed by Real-time PCR technology.The results showed that the expression level of PbFer2 gene increased in leaf with slight iron-deficiency,but it decreased when the iron-deficiency degree in leaf was increased." @default.
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- W2368694909 date "2013-01-01" @default.
- W2368694909 modified "2023-09-23" @default.
- W2368694909 title "Cloning and differential expression analysis of Fer2 gene in leaf of ‘Dangshansuli’ pear" @default.
- W2368694909 hasPublicationYear "2013" @default.
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