Matches in SemOpenAlex for { <https://semopenalex.org/work/W2368708396> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2368708396 abstract "Apyrase was isolated from soluble crude salivary glands extract of %Haemaphysalis longicornis% by a Cibacron Blue column and a Macrosphere weak cation-exchange column. Apyrase could hydrolyse ATP and ADP, but failed to hydrolyse AMP. The approach K m, obtained with ATP and ADP as substrates, were evaluated 0.2 μmol/L and the approach V max were 12.5 and 15.6 μmol/(min·mg) respectively. During the hydrolysis of ATP as monitored by HPLC, the UV-absorbing compounds observed were the ADP and AMP,and for ADP was the AMP only. Direct evidence showed that the hydrolysis site of ATP and ADP occurred by cleavage at the phosphate residues γ and β to the 5′ carbon atom of the ribose was obtained by enzymically hydrolyzing both [γ- 32 P] ATP and [β- 32 P] ADP and separating the products by ion-exchange HPLC. The mechanism of ATP and ADP hydrolysis is sequential removal of phosphate residues to produce, finally, AMP." @default.
- W2368708396 created "2016-06-24" @default.
- W2368708396 creator A5045466606 @default.
- W2368708396 date "2002-01-01" @default.
- W2368708396 modified "2023-09-25" @default.
- W2368708396 title "Purification and mechanism of enzymic hydrolysis of apyrase from salivary glands of Haemaphysalis longicornis" @default.
- W2368708396 hasPublicationYear "2002" @default.
- W2368708396 type Work @default.
- W2368708396 sameAs 2368708396 @default.
- W2368708396 citedByCount "0" @default.
- W2368708396 crossrefType "journal-article" @default.
- W2368708396 hasAuthorship W2368708396A5045466606 @default.
- W2368708396 hasConcept C141315368 @default.
- W2368708396 hasConcept C151730666 @default.
- W2368708396 hasConcept C173758957 @default.
- W2368708396 hasConcept C175156509 @default.
- W2368708396 hasConcept C179998833 @default.
- W2368708396 hasConcept C181199279 @default.
- W2368708396 hasConcept C185592680 @default.
- W2368708396 hasConcept C23265538 @default.
- W2368708396 hasConcept C2776127032 @default.
- W2368708396 hasConcept C2777132085 @default.
- W2368708396 hasConcept C2779420972 @default.
- W2368708396 hasConcept C2779508331 @default.
- W2368708396 hasConcept C28859421 @default.
- W2368708396 hasConcept C43369102 @default.
- W2368708396 hasConcept C43617362 @default.
- W2368708396 hasConcept C55493867 @default.
- W2368708396 hasConcept C59822182 @default.
- W2368708396 hasConcept C75385678 @default.
- W2368708396 hasConcept C83575541 @default.
- W2368708396 hasConcept C86803240 @default.
- W2368708396 hasConcept C94412978 @default.
- W2368708396 hasConceptScore W2368708396C141315368 @default.
- W2368708396 hasConceptScore W2368708396C151730666 @default.
- W2368708396 hasConceptScore W2368708396C173758957 @default.
- W2368708396 hasConceptScore W2368708396C175156509 @default.
- W2368708396 hasConceptScore W2368708396C179998833 @default.
- W2368708396 hasConceptScore W2368708396C181199279 @default.
- W2368708396 hasConceptScore W2368708396C185592680 @default.
- W2368708396 hasConceptScore W2368708396C23265538 @default.
- W2368708396 hasConceptScore W2368708396C2776127032 @default.
- W2368708396 hasConceptScore W2368708396C2777132085 @default.
- W2368708396 hasConceptScore W2368708396C2779420972 @default.
- W2368708396 hasConceptScore W2368708396C2779508331 @default.
- W2368708396 hasConceptScore W2368708396C28859421 @default.
- W2368708396 hasConceptScore W2368708396C43369102 @default.
- W2368708396 hasConceptScore W2368708396C43617362 @default.
- W2368708396 hasConceptScore W2368708396C55493867 @default.
- W2368708396 hasConceptScore W2368708396C59822182 @default.
- W2368708396 hasConceptScore W2368708396C75385678 @default.
- W2368708396 hasConceptScore W2368708396C83575541 @default.
- W2368708396 hasConceptScore W2368708396C86803240 @default.
- W2368708396 hasConceptScore W2368708396C94412978 @default.
- W2368708396 hasLocation W23687083961 @default.
- W2368708396 hasOpenAccess W2368708396 @default.
- W2368708396 hasPrimaryLocation W23687083961 @default.
- W2368708396 hasRelatedWork W1516120784 @default.
- W2368708396 hasRelatedWork W1556336658 @default.
- W2368708396 hasRelatedWork W1587164067 @default.
- W2368708396 hasRelatedWork W1637127028 @default.
- W2368708396 hasRelatedWork W1812007932 @default.
- W2368708396 hasRelatedWork W1967316507 @default.
- W2368708396 hasRelatedWork W1973543786 @default.
- W2368708396 hasRelatedWork W1978432279 @default.
- W2368708396 hasRelatedWork W1988602509 @default.
- W2368708396 hasRelatedWork W1988672208 @default.
- W2368708396 hasRelatedWork W1995501261 @default.
- W2368708396 hasRelatedWork W1997351782 @default.
- W2368708396 hasRelatedWork W2019464548 @default.
- W2368708396 hasRelatedWork W2022104864 @default.
- W2368708396 hasRelatedWork W2044702989 @default.
- W2368708396 hasRelatedWork W2056913009 @default.
- W2368708396 hasRelatedWork W2064621698 @default.
- W2368708396 hasRelatedWork W2066289410 @default.
- W2368708396 hasRelatedWork W2086276219 @default.
- W2368708396 hasRelatedWork W1988498481 @default.
- W2368708396 isParatext "false" @default.
- W2368708396 isRetracted "false" @default.
- W2368708396 magId "2368708396" @default.
- W2368708396 workType "article" @default.