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- W2368744516 abstract "Objective To optimize the extraction conditions of ephedrine in Ephedra sinica.Methods The extracts of Ephedra sinica were obtained by water decoction.Reversed high performance liquid chromatography-electrospray-time of flight tandem mass spectrometry(HPLC-ESI-TOF-MS) was used to determine the concentration of ephedrine in extracts through selected ion mode.Orthogonal design was employed to optimize the extraction procedure.Results The limit of detection was 0.05 μg·mL-1.The peak area and the concentration of ephedrine gave a linear relationship in the range of 0.005-0.5 mg·mL-1.The best method for extracting ephedrine in Ephedra sinica was water decoction.Using this approach,the optimized conditions were as follows:the process of 30 folds water,a value of 75 ℃ for heating temperature,a repeatability of 4 times and 40 min for each time.Conclusion HPLC-ESI-TOF-MS was proved to be sensitive,accuracy and suitable to determine ephedrine in complex matrix.Water decoction has the properties of being economic,environment friendly and having no pollution.It will probably be an alterative for extracting alkaloids such as ephedrine." @default.
- W2368744516 created "2016-06-24" @default.
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- W2368744516 date "2013-01-01" @default.
- W2368744516 modified "2023-09-26" @default.
- W2368744516 title "Optimization of the extraction conditions of ephedrine in Ephedra sinicaby HPLC-ESI-TOF-MS" @default.
- W2368744516 hasPublicationYear "2013" @default.
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