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- W2368751570 abstract "An optimal ISSR reaction system for Taiwania cryptomerioides was established with 25.0 μL reaction mixture made up of 40 ng genomic DNA,0.4 μmol/L primer,1.8 mmol/ L Mg2+,1.0 U Taq,0.2 mmol/L dNTP,and 2.5 μL 10×buffer.The PCR were performed as follows:4 min at 94℃,40 cycles of 45 s at 94℃,45 s at 51℃ to 56℃,and 2 min at 72℃,with a final extension of 7 min at 72℃.Ten polymorphic primers were screened using this reaction system.Twenty DNA samples from the wild population of T.cryptomerioides distributed in Lichuan City of Hubei Province were amplified by the 10 selected primers.The percentage of polymorphic bands was 61.97 %,Nei's gene diversity and Shannon's information measure were 0.136 9 and 0.216 0,respectively." @default.
- W2368751570 created "2016-06-24" @default.
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- W2368751570 date "2011-01-01" @default.
- W2368751570 modified "2023-09-26" @default.
- W2368751570 title "Establishment of ISSR system for the relict plant Taiwania cryptomerioides Hayata" @default.
- W2368751570 hasPublicationYear "2011" @default.
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