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- W2368752450 abstract "Objective To study the effection of multiplication capacity in vitro and anti-tumor features with defferent type and concentration monoclonal antibody CD3 treatment CIK cells,Optimize expansion projection.Methods Peripheral blood mononuclear cells were separated from many healthy blood with lymphocyte separating medium,CIK cells were induced to be generated and cultivated for 12 days with deferent type,concentration and combination of monoclonal antibody CD3(OKT3,UCHT1,HIT3a) and cytokine(γ-IFN,rhIL-2).Multiplication capacity of CIK cells were observed,activity of kill tumor cells were measured by MTT method.Results(1) Multiplication capacity with mixture of three deferent type monoclonal antibody CD3 was superior to only using monoclonal antibody CD3(P0.05).(2) Multiplication capacity and cytotoxicity of CIK cells were declined by increasing concentration of monoclonal antibody CD3.(3) Multiplication capacity and cytotoxicity of CIK cells were unchanged with deferent concentration rhIL-2.Conclusions Adding deferent type monoclonal antibody CD3 contributes to expansion in vitro when cultivating CIK cells,and provides high-performance immunocompetent cells for adoptive immunotherapy." @default.
- W2368752450 created "2016-06-24" @default.
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- W2368752450 date "2011-01-01" @default.
- W2368752450 modified "2023-09-25" @default.
- W2368752450 title "Influence of Different Cultivate Conditions on Cytokine-induced Killer Cells Expansion and Anti-tumor Features in vitro" @default.
- W2368752450 hasPublicationYear "2011" @default.
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