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- W2368754228 abstract "Objective To investigate effect of dendritic cells pulsed with tumor antigens which are used to treat with intracranial glioma.Methods dendritic cells were isolated from rat bone marrow precursors stimulated in vitro with granulocyte macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4).Then mature DCs were analyzed; Produce lysates and dendritic cells vaccinum; then we established animal model treated with dendritic cells vaccinum. The rat brains were then removed and examined histologically,and spleens were harvested for cell mediated cytotoxicity assays.Results Mature dendritic cells with typical cloved leaf shaped nucleus and long cytoplasmic dendrites, immunocytochemisty showed expressions of OX 6 and OX 62 were positive, cell mediated cytotoxicity assays showed that there were statistically significant compared with control cells. At necropsy, the size of neoplasm is larger in tumor than control group ( P 0.01 ). Conclusion A method to generate large number of dendritic cells from rat bone marrow in vitro and manufacture dendritic cells vaccinum is established, and dendritic cells pulsed with lysate could inhibit significantly the growth of tumor; which may contribute to further study on the role of them in the immuntherapy of brain glioma." @default.
- W2368754228 created "2016-06-24" @default.
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- W2368754228 date "2004-01-01" @default.
- W2368754228 modified "2023-09-24" @default.
- W2368754228 title "Experimental Study of Vaccination with Dendritic Cells Pulsed with Tumor Cell Lysates for Immunotherapy of C_6 Glioma" @default.
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