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- W2368755527 abstract "Mature peptide of chicken IFN-α gene was amplified from activated chicken peripheral blood lymphocytes by PCR.pET32a-α and pGEX6p-α were constructed for IFN-α expression.Purified pET32a-α induced protein was injected into mice for peptide-specific antibody which was detected by pGEX6p-α induced protein.As a result,amplification of mature peptide of chicken IFN-α gene could be successfully expressed in prokaryotic expression vector for immunized mice to produce specific antibody,the antibody titer was 1∶12 000.The results provide the foundation for the preparation of safe and efficient recombinant interferon and a more reliable technique for the quantitative detection of the body producing IFN-α which can reflect the immune status of the body against pathogens,as well as a detection method for some other biomolecules with no commercial antibodies." @default.
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- W2368755527 date "2013-01-01" @default.
- W2368755527 modified "2023-09-27" @default.
- W2368755527 title "Prokaryotic expression of chicken α-interferon mature peptide and its preparation of polyvalent serum." @default.
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