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- W2368755754 abstract "The high quality genome total DNA of Inonotus obliquus extracted fast through the CTAB law,was used in ISSR amplification.And the optimal 25 μL ISSR reaction system of Inonotus obliquus was determined.The template DNA density was 15 ng·μL-1,dNTPs was 150 μmol·L-1,the primer density was 25 μmol·L-1,the Taq DNA polymerase was 2.0 U,the Mg2+ concentration was 1.4 mmol·L-1,the 10×buffer density is 2.5 μL,and the rest are supplemented with the double-distilled water.And the procedures of ISSR amplification are 5 min at 94℃,followed by 35 cycles of 1 min at 94℃,1 min at 45℃~51℃(depends on the primers),1 min at 72℃,and last extension at 72℃ for 5 min.The amplified products were reserved at 4℃.Based on these reaction conditions,16 ISSR primers were screened out and used in the further research.ISSR-PCR reactions of 21 Inonotus obliquus strains were conducted successfully with the using of primer UBC842." @default.
- W2368755754 created "2016-06-24" @default.
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- W2368755754 date "2012-01-01" @default.
- W2368755754 modified "2023-09-28" @default.
- W2368755754 title "Establishment and Optimization of ISSR-PCR Reaction System of Inonotus obliquus" @default.
- W2368755754 hasPublicationYear "2012" @default.
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