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- W2368759357 abstract "[Objective] The purpose of the study was to set up the high efficient plantlet regeneration system of Malus pumila var.niedzwetzkyana (Dieck) Schneid.[Method] With shoot tips and stem segments with axillary buds of niedzwet-zkyana as explants,the optimum sterilization time,basic medium,proliferation medium and rooting medium were screened out through tissue culture experiments.[Result] As the sterilization time was extended,the contamination rate of explants was decreased obviously,but the damage on explants was increased significantly.The explants sterilized with 0.1% mercuric chloride for 5 min grew normally and their contamination rate was only 3.3%.In the proliferation media MS+6-BA 0.5 mg/L +NAA 0.1 mg/L,MS+6-BA 0.5 mg/L+NAA 0.2 mg/L and MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,each explant proliferated 4.1,6.8 and 3.3 adventitious buds averagely.In the rooting media 1/2MS+0.1 mg/L IBA+0.1 mg/L NAA,1/2MS+0.2 mg/L IBA+0.2 mg/L NAA and 1/2MS+0.5 mg/L IBA+0.5 mg/L NAA,the rooting rates of seedlings were 56.7%,96.7% and 83.3% resp.[Conclusion] This study laid a good foundation for the rapid mass propagation in vitro,industrial seedling-raising and landscape application of niedzwet-zkyana." @default.
- W2368759357 created "2016-06-24" @default.
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- W2368759357 date "2009-01-01" @default.
- W2368759357 modified "2023-09-23" @default.
- W2368759357 title "Tissue Culture of Malus pumila var. niedzwetzkyana (Dieck) Schneid" @default.
- W2368759357 hasPublicationYear "2009" @default.
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