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- W2368761392 abstract "ttβGLY is a thermostable β-glucosidase from thermus thermophilus.This β-glucosidase has high activity of hydrolysis and transgalactosylation.The optimized fermentation compositions of genetic engineered Escherichia coli producing thermostable β-glucosidase are as following: medium composition: Peptone 14 g/L,yeast powder 7 g/L,whey 13.3 g/L,NaCl 10 g/L,KH2PO4 3 g/L,K2HPO4 5 g/L,MgSO4·7H2O 1 g/L;Seed medium composition: Peptone 10 g/L,yeast powder 5 g/L,glucose 4 g/L,NaCl 10 g/L,CaCO3 5 g/L.Under the condition of inoculom age of 12 h,inoculom volume of 8%,triangular flask with the amount of 250 mL while shaking flask is 40 mL and fermentation time of 24 h at 37 ℃,cell dry weight is 10.010 g/L and enzyme activity is 2.384 U/mL,showing 4.4 fold and 2.4 fold improvement over that of the initial condition." @default.
- W2368761392 created "2016-06-24" @default.
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- W2368761392 date "2013-01-01" @default.
- W2368761392 modified "2023-09-23" @default.
- W2368761392 title "Optimization of fermentation condition of recombinant Escherichia coli producing thermostable β-glucosidase" @default.
- W2368761392 hasPublicationYear "2013" @default.
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