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- W2368774114 abstract "OBJECTIVE To study the effects of sucrose concentration,the proportion of NH+4and NO-3 and initial total nitrogen content in the MS culture medium on the growth of suspension cell and accumulation of periplocin and 4-methoxysalicylaldehyde.METHODS The sucrose concentration gradient was set up as 1%,2%,4% and 5%,with 3% sucrose as control.The proportions of NH+4 and NO-3 were 0∶1,1∶0,2∶1,1∶1 and 1∶2 in five groups.Total nitrogen content of control group was 60 mmol·L-1 in MS minimal medium.The total nitrogen contents of experimental groups were 1/4,1/2 and 2 times as that of the control.RESULTS 4% sucrose was in favor of the growth of cell.5% sucrose benefited synthetizing the ingredient of periplocin and 3% sucrose benefited synthetizing the ingredient of 4-methoxysalicylaldehyde.4% sucrose was in favor of the increase of the output of periplocin and 4-methoxysalicylaldehyde.It was beneficial to increase the growth of cell and compose the ingredients of periplocin and 4-methoxysalicylaldehyde while the proportion of NH+4 and NO-3 was 1 to 2.60 mmol·L-1 total nitrogen content was good for the growth of cell.30 mmol·L-1 total nitrogen content improved the content and output of the ingredient of periplocin and 4-methoxysalicylaldehyde.CONCLUSION The sucrose concentration,the proportion of NH+4 and NO-3,and total nitrogen content can affect on the growth of cell and the accumulation of the ingredients of periplocin and 4-methoxysalicylaldehyde." @default.
- W2368774114 created "2016-06-24" @default.
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- W2368774114 date "2011-01-01" @default.
- W2368774114 modified "2023-09-25" @default.
- W2368774114 title "Studies on Sucrose Concentration and Nitrogen Source in the System of Cell Suspension Culture of Periploca sepium Bunge" @default.
- W2368774114 hasPublicationYear "2011" @default.
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