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- W2368792290 abstract "Objective To establish a method for genomic DNA extraction of Marchantia convoluta; to establish and optimize the ISSR-PCR reaction system for Marchantia convoluta.Methods A modified CTAB method was applied to extract genomic DNA from Marchantia convoluta and the orthogonal design was applied to optimize ISSR-PCR reaction system in three levels of four factors( Taq DNA polymerase,Mg2 +,dNTP,primer).Results The effects of the improved CTAB method worked well,and the purity of DNA was high.An optimum ISSR-PCR reaction system was established for Marchantia convoluta and the 20 μl ISSR-PCR system contained 50ng of the template DNA,2.0 μl of PCR buffer,0.5 U of Taq polymerase,250μmol /L dNTP mix,2.0mmol /L of Mg2 +,0.6 μmol/L of primers.Conclusion a method for genomic DNA extraction of Marchantia convoluta was established and the ISSR-PCR reaction system was optimized." @default.
- W2368792290 created "2016-06-24" @default.
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- W2368792290 date "2014-01-01" @default.
- W2368792290 modified "2023-09-28" @default.
- W2368792290 title "Establishment and optimization of genomic DNA extraction and ISSR-PCR reaction system for Marchantia convoluta" @default.
- W2368792290 hasPublicationYear "2014" @default.
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