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- W2368800552 abstract "Mature microRNAs(miRNAs) are a class of non-coding RNAs with about 21 nucleotides in length. The majority of miRNA matches perfectly to 3' UTR mRNA sequences and results in mRNA degradation. They also regulate target genes by binding imperfectly to mRNA sequences and attenuate translation. In this study, let-7b and let-7c were cloned and sequenced by using the methods of small RNA cDNA libraries, ploy(A)-tail and primer-extend RT-PCR from porcine skeletal muscle tissues. The results showed that the two methods for direct cloning miRNA were highly specific, and RNA secondary structure prediction revealed that the longer sequences containing the miRNA could fold steadily stem-loop structure. These two miRNAs were firstly experimentally identified from pig, offering new approaches in miRNA identification." @default.
- W2368800552 created "2016-06-24" @default.
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- W2368800552 date "2008-01-01" @default.
- W2368800552 modified "2023-09-26" @default.
- W2368800552 title "Cloning of Porcine miRNA let-7b and let-7c by Two Methods" @default.
- W2368800552 hasPublicationYear "2008" @default.
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