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- W2368803054 abstract "Single-molecule detection is the ultimate sensitivity in analytical chemistry and has been largely unavailable in electrochemical analysis. Here, we demonstrate the feasibility of detecting electrochemically inactive single biomacromolecules, such as enzymes, antibodies, and DNA, by blocking a solution redox reaction when molecules adsorb and block electrode sites. By oxidizing a large concentration of potassium ferrocyanide on an ultramicroelectrode (UME, radius ≤150 nm), time-resolved, discrete adsorption events of antibodies, enzymes, DNA, and polystyrene nanospheres can be differentiated from the background by their footprint. Further, by assuming that the mass transport of proteins to the electrode surface is controlled mainly by diffusion, a size estimate using the Stokes-Einstein relationship shows good agreement of electrochemical data with known protein sizes." @default.
- W2368803054 created "2016-06-24" @default.
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- W2368803054 date "2015-06-29" @default.
- W2368803054 modified "2023-10-17" @default.
- W2368803054 title "Observation of Single-Protein and DNA Macromolecule Collisions on Ultramicroelectrodes" @default.
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- W2368803054 doi "https://doi.org/10.1021/jacs.5b04545" @default.
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