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- W2368817188 abstract "A novel derivatization method for the determination of amino acids has been developed by use of o-nitrobenzenesulfonyl chloride.The optimized conditions of derivatization were as following:the reaction temperature 25 ℃;the pH value 9 and the reaction time 10 min.The chromatographic conditions were as following:C18 column(5 μm,4.6 mm×250 mm),the detection wavelength 275 nm,flow rate 1.0 mL·min-1,mobile phase A:30 mmol/L NH4Ac,mobile phase B:ACN,gradient elution.The calibration curves for 20 amino acids were linear ranging from 0.025 to 6.4 mmol/L.The linear correlation coefficients were between 0.998 0 and 0.999 8.The limits of detection were 0.01-0.113 nmol.The method was simple,rapid and reliable,and suitable for the derivatization of amino acids and other substances which have amino group." @default.
- W2368817188 created "2016-06-24" @default.
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- W2368817188 date "2008-01-01" @default.
- W2368817188 modified "2023-09-22" @default.
- W2368817188 title "Determination of Amino Acids by High Performance Liquid Chromatography with o-Nitrobenzenesulfonyl Chloride as Derivatization Reagent" @default.
- W2368817188 hasPublicationYear "2008" @default.
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