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- W2368840941 abstract "The MalQ gene was amplified from Streptomyces ST66 by PCR, cloned into pTrc-CKS and sequenced. MalQ sequence was analyzed by BLAST and displayed high similarity(97%) to MalQ gene of Streptomyces coelicolor reported in GenBank. The recombined plasmid pTrc- MalQ was transformed into Escherichia coli Top 10F' and induced by IPTG. MalQ gene and CKS(CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)gene fusion protein(molecular weight:about 106 kD) could be detected by SDS-PAGE gel electrophoresis. The crude enzyme was demonstrated having 4-glucanotransferase activity with thin layer chromatography." @default.
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- W2368840941 date "2007-01-01" @default.
- W2368840941 modified "2023-09-24" @default.
- W2368840941 title "Cloning and Fusion Expression of the MalQ Gene from Streptomyces ST66" @default.
- W2368840941 hasPublicationYear "2007" @default.
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