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- W2368863821 abstract "The upstream regulatory region of the tapetal specific gene A9 was amplified from Arabidopsis thaliana genome by polymerase chain reaction and cloned into HincⅡ and KpnⅠ site of pUC18. Sequence analysis showed that the cloned fragment contained 364 nucleotides, and shared a sequence homology of 99.2% with the reported A 9 promoter. The putative TATA box was present at position -76 to -69. Two motifs, TGTGG and TGTGA, which were considered to be responsible for the specificity and enhancement of gene expression in the tapetal, were found within -291 to -287 and -243 to -239 region respectively. Gene construct that contained the β glucuronidase ( GUS ) gene under the control of A 9 promoter or CaMV 35 s promoter was introduced into anthers and calli of maize by particle bombardmend. The GUS activity was detected only in anthers by fluorometric assay." @default.
- W2368863821 created "2016-06-24" @default.
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- W2368863821 date "2000-01-01" @default.
- W2368863821 modified "2023-09-25" @default.
- W2368863821 title "Isolation and Sequencing Analysis of the Promoter for an Anther specific Gene from Arabidopsis thaliana" @default.
- W2368863821 hasPublicationYear "2000" @default.
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