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- W2368872885 abstract "Objective To analyze the difference of GRA1 gene from RH and B36 strains of Toxoplasma gondii.Methods The specific fragment of GRA1 gene was obtained by PCR from RH and B36 strains of Toxoplasma gondii.The fragments of PCR products were cloned into a high level sequencing vector pMD-18- T, then transferred into JM109,a recombinant pDM-18- T/GRA1 was constructed,it was identified by PCR.The DNA sequence analysis was done by DNA star software.Results The recombinant pDM- 18- T/GRA1 from RH and B36 strains of Toxoplasma gondii was constructed. After homology analysis,the specific fragment of GRA1 gene from RH and B36 strains of Toxoplasma gondii showed 99% homology with RH strain from GenBank (No. of sequence:EU983103.1).The two specific fragments of GRA1 genes from RH and B36 strains of Toxoplasma gondii shared 100% homology by DNA star software.Conclusions There is no difference in the sequences of the GRA1 gene between from RH and B36 strains of Toxoplasma gondii. The GRA1 gene of Toxoplasma gondii is highly conservative." @default.
- W2368872885 created "2016-06-24" @default.
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- W2368872885 date "2009-01-01" @default.
- W2368872885 modified "2023-09-24" @default.
- W2368872885 title "Cloning and Sequence Analysis of GRA1 Gene from RH and B36 Strains of Toxoplasma Gondii" @default.
- W2368872885 hasPublicationYear "2009" @default.
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