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- W2368878292 abstract "【Objective】To determine whether seeds of Malus robusta Rehd.are infected by apple latent virus and to screen the efficient elimination method,【Method】the multiplex RT-PCR system were used to detect apple latent virus in the seeds of Malus robusta Rehd.,and to evaluate the effect of four treatments(Hot water,NaOH,KMnO4and Na3PO4) on the virus elimination.【Result】The results showed that seeds of Malus robusta Rehd.were infected by ASGV and ACLSV but not by ASPV,and the infection rates were 28% and 9% respectively,while the coinfecton rate was only 4%.The effects of four treatments on virus elimination were different.After seeds soaked for 30 minutes in hot water and 2% sodium hydroxide solution,1% potassium permanganate solution and 10% trisodium phosphate solution,ACLSV completely disappeared.But the elimination rate of ASGV varied with treatments.【Conclusion】The elimination efficiency of ASGV was 42% when the seeds were treated by 1% potassium permanganate solution and 10% trisodium phosphate solution,and 82% of virus elimination efficiency was achieved by the treatment with 2% sodium hydroxide solution.But the hot water treatment had no effect on ASGV." @default.
- W2368878292 created "2016-06-24" @default.
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- W2368878292 date "2013-01-01" @default.
- W2368878292 modified "2023-10-03" @default.
- W2368878292 title "Detection of apple latent virus in seeds of Malus robusta and effect of physicochemical treatment on the virus" @default.
- W2368878292 hasPublicationYear "2013" @default.
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