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- W2368902639 abstract "Objective To establish a HPLC method for determination of nuciferine contents in space lotus leaf,and determine the content of nuciferine at different growth stages and harvest time.Methods The Hypersil BDS C18 column(4.6 mm×250 mm,5.0 μm) was used with acetonitrile-water-triethylamine-galcial acetic acid(27:70.6:1.6:0.78) as mobile phase,the flow rate was 1.0 mL/min and the UV detection wavelength was 270 nm.Results Under the chromatographic conditions,nuciferine was well separated and showed a good linear relationship at the range of 0.01 -0.2 mg/mL.The contents of nuciferine were significantly different at different growth stages and different harvest time,and were highest at early august in newborn leaves.Conclusion The content of nuciferine in nearly mature leaves was more than that in mature leaves.The content of nuciferine in nearly mature leaves at august and septemper was more than that at other month,so that the nearly mature leaves at august and septemper were best for harvest.These results may be useful for the development and utilization of Lotus leaf resources." @default.
- W2368902639 created "2016-06-24" @default.
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- W2368902639 date "2013-01-01" @default.
- W2368902639 modified "2023-09-23" @default.
- W2368902639 title "Detemination of nuciferine in space lotus leaf at different growth stages and harvest time by HPLC" @default.
- W2368902639 hasPublicationYear "2013" @default.
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