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- W2368931477 abstract "A simple and reliable SHR rat ventricular myocytes isolation method was explored and established to improve cell survival and the success rate of patch-clamp experiments.In present study,the isolation method of rats ventricular myocytes was conducted to investigate for the patch clamp experiment and observed the L-type calcium channel current.The Langendorff perfusion method was used to isolate the ventricular myocytes from SHR rats.The whole-cell patch clamp technique was used to record the calcium current(ICa-L) of beingisolated ventricular myocytes from SHR rats.The observation of cells showed that this method was well-constructed,rod-like and the cross striation could be observed clearly.The typical L-type calcium current was recorded by the whole-cell patch clamp recording.The activation potential was-40 mV;peak potential was 0 mV;reversal potential was +50-+60 mV;Meamwhile it was in the voltage-dependent manner.The L-type calcium current observed could be inhibited by the calcium channel blocker-Verapamil.The rats ventricular myocyte samples which were isolated and prepared by this method would be suitable for the patch clamp electrophysiologicalobservation." @default.
- W2368931477 created "2016-06-24" @default.
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- W2368931477 date "2010-01-01" @default.
- W2368931477 modified "2023-09-24" @default.
- W2368931477 title "Improved method for isolation of SHR rats ventricular myocytes and observation of L-type calcium current" @default.
- W2368931477 hasPublicationYear "2010" @default.
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