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- W2368969095 abstract "[Objective] The aim of the research was to discuss a kind of method for extracting high-quality DNA from Thellungiella salsuginea.[Method] DNA was extracted from T.salsuginea by simplified SDS method,improved CATB method,CTAB method and improved urea method respectively.The purity and concentration of DNA extracted by different methods were determined.And they were detected by electrophoresis and restriction enzyme digestion and their results were compared.[Result] DNA extracted by improved CTAB method had high purity and good quality,without the pollution of carbohydrate,phenols and protein.It could be used in PCR amplification and digestion with restriction enzymes and the improved CTAB method was more accordant with the demands of molecular biology research than other methods.DNA extracted by CTAB method was not polluted by protein,but it was polluted by phenols.It could be taken as the templates for PCR amplification.The quality of DNA extracted by SDS method and urea method was so worse that it could not meet with the demands of molecular biology research.[Conclusion] Compared with other methods,the improved CATB method was more suitable for molecular biology research." @default.
- W2368969095 created "2016-06-24" @default.
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- W2368969095 date "2008-01-01" @default.
- W2368969095 modified "2023-09-23" @default.
- W2368969095 title "Comparison of Several Methods of Extracting Trace DNA from Thellungiella salsuginea" @default.
- W2368969095 hasPublicationYear "2008" @default.
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