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- W2368989822 abstract "Objective To observe the ultrastructural features of lipid storage myopathy and explore the diagnostic value of electron microscopy in lipid storage myopathy and the relationship between this disease and mitochondrial myopathy.Methods Muscle biopsies were performed in 7 patients who had weakness.Quadriceps and gastrocnemius muscles were obtained from biopsy and processed with routine electron microscopic procedures.Semithin sections were made with ultramicrotome and stained with buffered toluidine blue.After the proper positions were selected,ultrathin sections were made and stained with uranyl acetate and lead citrate.A JEM 2000EX transmission electron microscope was used in observation and analysis.Results A lot of lipid droplets were found in muscle cells of all cases.Lipid droplets arranged tightly as a string of beads in the residual myofilaments and fused each other.Abnormal mitochondrias with crystalline intramitochondrial inclusion bodies were found in one case.The intracellular lipid droplets were also found in endothelium of capillaries and fibroblasts of interstitial tissue.The obvious neuralgia occurred in one case,whose electron microscopy results presented that the myelin lamellae of myelin sheath was abnormally widened and lipid droplets presented in some Schwann cells.Proliferation of collagen fibers and regenerating muscle cells appeared in some cases.ConclusionLipid storage myopathy is a group of myopathies with unique ultrastructural characteristics and is related with mitochondrial disorders.It is useful for definitive diagnosis of the disease to perform electron microscopy." @default.
- W2368989822 created "2016-06-24" @default.
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- W2368989822 date "2008-01-01" @default.
- W2368989822 modified "2023-09-23" @default.
- W2368989822 title "Diagnosis of lipid storage myopathy by electron microscopy" @default.
- W2368989822 hasPublicationYear "2008" @default.
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