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- W2369041488 abstract "Objective To establish a new method for rapid detection of common Staphylococci based on liquichip system.Methods Five species of Staphylococci were detected by symmetrical PCR and unsymmetrical PCR. A pair of general primers based on highly conserved segment of 16 S rRNA gene was designed for the above PCR. The amplified products of 5 species of Staphylococci were sequenced and detected by applying liquichip system.Results The highly conserved segments of 16 S rRNA gene from 5 species of Staphylococci were amplified by applying two different PCR methods (symmetrical PCR and unsymmetrical PCR), and the products were 780-820 bp in length. The unsymmetrical PCR produced a great quantity of single-strand nucleotides. The hybridization signal of unsymmetrical PCR products was stronger than that of symmetrical PCR products in liquichip system. The identificating rate of liquichip system for 5 species of Staphylococci accounted for 100%.Conclusion 16 S rRNA gene may be used as the target sequence of rapid identification for bacteria. The unsymmetrical PCR products can significantly enhance the hybridization sensibility. Liquichip system is characteristic of simply, rapid, high-throughput and high-performance identification for bacteria and may be further developed as a new method of bacterial identification." @default.
- W2369041488 created "2016-06-24" @default.
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- W2369041488 date "2008-01-01" @default.
- W2369041488 modified "2023-09-23" @default.
- W2369041488 title "Experimental study on rapid detection of common Staphylococci based on liquichip system" @default.
- W2369041488 hasPublicationYear "2008" @default.
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