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- W2369104080 abstract "To establish a simple, rapid and precise method to determine platelet serotonin.Serotonin (5-hydroxytryptamine; 5-HT) was determined in platelet pellet after simple deproteinization, following ion-pair high performance liquid chromatography (HPLC) on reverse-phase column: Nova-pak C18 column (150 mm x 4.6 mm, 4 microm), serotonin and internal standard (N-methylserotonin) were detected by fluorometry.The intra-day CV was 2.29% and inter-day CV was 3.88%. The mean recovery of serotonin in platelet was 97.5%, the linear range was 20 - 20000 nmol/ L, and the detection limit was 1 nmol/L. 5-hydroxyindole acetic acid (5-HIAA), Trp, Phe and Tyr had no interference with the chromatographic condition used in this trial.The metod described here is simple, convenient, rapid, sensitive, and accurate for clinical and scientific research." @default.
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- W2369104080 date "2004-06-01" @default.
- W2369104080 modified "2023-09-26" @default.
- W2369104080 title "[Determination of platelet serotonin by high performance liquid chromatography with fluorometric detector]." @default.
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