Matches in SemOpenAlex for { <https://semopenalex.org/work/W2369124258> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2369124258 abstract "【Objective】 To investigate the effect of silencing caspase-3 gene on apoptosis of rat MSC under hypoxia and serum deprivation in vitro. 【Methods】 Lentiviral shRNA interference vector targeting caspase-3 was constructed and transfected into MSC. Cells were treated with hypoxia and serum deprivation. The apoptosis were evaluated by flow cytometry and Hoechst fluorochrome staining. The expression of caspase-3 protein was detected by Western blot analysis. The expression of caspase-3,Bcl-2 and Bax mRNA were examined by Real-time PCR. 【Results】 Recombinant lentivirus was transfected into MSC successfully. The apoptotic rate in MSC-shRNA,MSC-vector and MSC were(13.66 ± 0.20)%,(21.86 ± 0.43)% and(22.28 ± 0.48)%(P 0.01), respectively. Compared with control groups, silencing caspase-3 down-regulated the mRNA level of caspase-3, up-regulated the mRNA level of Bcl-2 and down-regulated the mRNA level of Bax and the ratio of Bcl-2 to Bax increased(P 0.05).【Conclusions】 Silencing caspase- 3 improves the ability of survival of MSC under hypoxia and serum deprivation in vitro." @default.
- W2369124258 created "2016-06-24" @default.
- W2369124258 creator A5034883142 @default.
- W2369124258 date "2013-01-01" @default.
- W2369124258 modified "2023-09-23" @default.
- W2369124258 title "Effect of Silencing Caspase-3 Gene on Apoptosis of Rat Bone Marrow Mesenchymal Stem Cells under Hypoxia and Serum Deprivation" @default.
- W2369124258 hasPublicationYear "2013" @default.
- W2369124258 type Work @default.
- W2369124258 sameAs 2369124258 @default.
- W2369124258 citedByCount "0" @default.
- W2369124258 crossrefType "journal-article" @default.
- W2369124258 hasAuthorship W2369124258A5034883142 @default.
- W2369124258 hasConcept C104317684 @default.
- W2369124258 hasConcept C119056186 @default.
- W2369124258 hasConcept C12519072 @default.
- W2369124258 hasConcept C153911025 @default.
- W2369124258 hasConcept C173396325 @default.
- W2369124258 hasConcept C178790620 @default.
- W2369124258 hasConcept C185592680 @default.
- W2369124258 hasConcept C190232843 @default.
- W2369124258 hasConcept C190283241 @default.
- W2369124258 hasConcept C198826908 @default.
- W2369124258 hasConcept C2776415932 @default.
- W2369124258 hasConcept C31573885 @default.
- W2369124258 hasConcept C540031477 @default.
- W2369124258 hasConcept C54009773 @default.
- W2369124258 hasConcept C54355233 @default.
- W2369124258 hasConcept C553184892 @default.
- W2369124258 hasConcept C55493867 @default.
- W2369124258 hasConcept C7836513 @default.
- W2369124258 hasConcept C81885089 @default.
- W2369124258 hasConcept C86803240 @default.
- W2369124258 hasConcept C95444343 @default.
- W2369124258 hasConceptScore W2369124258C104317684 @default.
- W2369124258 hasConceptScore W2369124258C119056186 @default.
- W2369124258 hasConceptScore W2369124258C12519072 @default.
- W2369124258 hasConceptScore W2369124258C153911025 @default.
- W2369124258 hasConceptScore W2369124258C173396325 @default.
- W2369124258 hasConceptScore W2369124258C178790620 @default.
- W2369124258 hasConceptScore W2369124258C185592680 @default.
- W2369124258 hasConceptScore W2369124258C190232843 @default.
- W2369124258 hasConceptScore W2369124258C190283241 @default.
- W2369124258 hasConceptScore W2369124258C198826908 @default.
- W2369124258 hasConceptScore W2369124258C2776415932 @default.
- W2369124258 hasConceptScore W2369124258C31573885 @default.
- W2369124258 hasConceptScore W2369124258C540031477 @default.
- W2369124258 hasConceptScore W2369124258C54009773 @default.
- W2369124258 hasConceptScore W2369124258C54355233 @default.
- W2369124258 hasConceptScore W2369124258C553184892 @default.
- W2369124258 hasConceptScore W2369124258C55493867 @default.
- W2369124258 hasConceptScore W2369124258C7836513 @default.
- W2369124258 hasConceptScore W2369124258C81885089 @default.
- W2369124258 hasConceptScore W2369124258C86803240 @default.
- W2369124258 hasConceptScore W2369124258C95444343 @default.
- W2369124258 hasLocation W23691242581 @default.
- W2369124258 hasOpenAccess W2369124258 @default.
- W2369124258 hasPrimaryLocation W23691242581 @default.
- W2369124258 hasRelatedWork W2333977052 @default.
- W2369124258 hasRelatedWork W2364546322 @default.
- W2369124258 hasRelatedWork W2389016740 @default.
- W2369124258 hasRelatedWork W2402293239 @default.
- W2369124258 hasRelatedWork W2405407799 @default.
- W2369124258 hasRelatedWork W2405815479 @default.
- W2369124258 hasRelatedWork W2410876256 @default.
- W2369124258 hasRelatedWork W2416638577 @default.
- W2369124258 hasRelatedWork W2417997257 @default.
- W2369124258 hasRelatedWork W2418263754 @default.
- W2369124258 hasRelatedWork W2464136916 @default.
- W2369124258 hasRelatedWork W2514982668 @default.
- W2369124258 hasRelatedWork W2578718647 @default.
- W2369124258 hasRelatedWork W2787430223 @default.
- W2369124258 hasRelatedWork W3006325446 @default.
- W2369124258 hasRelatedWork W3028735483 @default.
- W2369124258 hasRelatedWork W3028926320 @default.
- W2369124258 hasRelatedWork W3031891190 @default.
- W2369124258 hasRelatedWork W310708657 @default.
- W2369124258 hasRelatedWork W3149412656 @default.
- W2369124258 isParatext "false" @default.
- W2369124258 isRetracted "false" @default.
- W2369124258 magId "2369124258" @default.
- W2369124258 workType "article" @default.