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- W2369252783 abstract "The effects of in vitro cultural conditions on in vitro development of Yan-Bian cattle oocytes were examined in this study.Bovine oocytes obtained from 2- to 8-mm ovarian follicles at slaughter were matured,fertilized and cultured in vitro.In TCM199 group the rates of cleavage(56.3%) were significantly higher than in D-PBS group(33.6%)(P0.01),but the percentages of developing to blastocysts and hatched blastocysts were no significant difference in TCM199 group(15.1%,13.7%) and D-PBS(10.5%,8.7%)(P0.05).The rates of developing to blastocysts and hatching stage were no significant difference(P0.05) between bovine oocytes matured in the TCM199 medium supplemented with 10% FBS and 10 mg/L LH,1 mg/L 17-β estradiol(17.5%,15.3%) and control group(12.1%,10.5%).The proportion of embryos that cleavage reached the blastocyte and hatched blastocysts in co-culture with cumulus cell monolayer(78.0%,11.5%,9.9%) were significantly higher than in control group(68.1%,5.4%,3.6%)(P0.01)." @default.
- W2369252783 created "2016-06-24" @default.
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- W2369252783 date "2004-01-01" @default.
- W2369252783 modified "2023-09-25" @default.
- W2369252783 title "Effects of in vitro Cultural Condition on in vitro Development of Yan-Bian Yellow Cattle Oocytes" @default.
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