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- W2369258716 abstract "In view of the features that industrial gibberellin-producing strains are all multi-cellular hyphaethe and producing no spores,firstly the gibberellin-mycelium individual free protoplasts was made by means of enzymolysis for the cell wall,secondly a model was established to obtain gibberellin high-yielding strain by eliminating feedback inhibition and repression of high concentrations of reaction substrates and endogenous gibberellin and increasing the enzyme activity of gibberellin biosynthesis.By the use of the model established,N7298 and other drug-resistant strains of protoplast regeneration of high-yielding mutant strains were successfully obtained.The shake flask titer of N7298 increased 23.4% than that of starting strain N,and its production level had been increased by 24.1% in the 20 m3 tank." @default.
- W2369258716 created "2016-06-24" @default.
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- W2369258716 date "2012-01-01" @default.
- W2369258716 modified "2023-09-25" @default.
- W2369258716 title "Establishment and Application of A Model of Protoplast Physiochemical Mutation and Directional Selection for Gibberellin-Producing Strain" @default.
- W2369258716 hasPublicationYear "2012" @default.
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