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- W2369286659 abstract "Objective To investigate the effect of human adipose derived mesenchymal stems cells(haMSC)on T lymphocytes of aplastic anemia patients(AA)and to explore its possible mechanism. Methods HaMSCs were ex-tracted from human adipose tissues. T lymphocytes were isolated from peripheral blood of patients with AA by density gradient centrifμgation. HaMSCs were co-cultured with T-lymphocytes. Inhibitory effect of haMSCs on AA-TLCs was measured by MTT assay,T-bet and GATA-3 level were examined by real-time PCR and Western blot. The level of INF-γ,IL-2,IL-4 and IL-10 was detected by ELASA. Results HaMSCs showed inhibitory effect on lymphocyte pro-liferation of aplastic anemia patients in a time dependent manner:After 3,5 and 7 days' co-culturing,their inhibitory rates were 34. 94% ± 10. 11%,53. 44% ± 6. 16%,68. 13% ± 15. 24%,respectively(P 0. 05). The T-bet mRNA and protein levels in MSCs + AA-TLCs group were significantly lower than that in AA-TLCs group,while the GATA-3 mRNA and protein levels were significantly higher than that in AA-TLCs group. The concentrations of Th1 type cy-tokins INF-γ and IL-2 in MSCs + AA-TLCs group[(8. 7 ±2. 7)ng/L and(7. 3 ±1. 3)ng/L,respectively]were sig-nificantly lower than those in AA-TLCs group[(41. 5 ± 3. 7)ng / L and(61. 5 ± 8. 7)ng / L,respectively(P 0. 05)]. But the concentrations of Th2 type cytokins IL-4 and IL-10 in MSCs + AA-TLCs group[(88. 6 ± 15. 2)ng / L and(38. 3 ± 11. 8)ng / L,respectively]were significantly higher than those in AA-TLCs group[(1. 4 ± 0. 6)ng/L and(1. 3 ±0. 2)ng/L,respectively(P 0. 05)]. Conclusions HaMSCs can regulate T lymphocytes of aplastic anemia patients in vitro,which was related with the depression of Th1-dominant response due to the dis-order of T-bet and GATA-3 gene expression." @default.
- W2369286659 created "2016-06-24" @default.
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- W2369286659 date "2014-01-01" @default.
- W2369286659 modified "2023-09-25" @default.
- W2369286659 title "Immune-modulatory effect of human adipose derived mesenchymal stem cells on T lymphocytes of patients with aplastic anemia" @default.
- W2369286659 hasPublicationYear "2014" @default.
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