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- W2369291109 abstract "Secretory proteins in the oesophageal gland cells of plant-parasitic nematodes have key roles in nematodes parasitism of plant.A new venom allergen-like protein gene(Dd-vap-1) was cloned from Ditylenchus destructor by RT-PCR and RACE.The full length cDNA of Dd-vap-1 was 1 346 bp.The cDNA sequence consisted of a 1 209 bp open reading frame encoding 402 amino acid residues that were franked by a 69 bp 5'-untranslated region and a 68 bp 3'-untranslated region(GenBank accession No.JQ341057).The theoretical molecular weight and isoelectric point of the putative protein were 45.3 kD and 6.68,respectively.The sequencing analysis showed that this gene was classified as a member of venom allergen-like protein.In situ hybridization analysis showed that the transcript of Dd-vap-1 accumulated exclusively within the subventral oesophageal cell of D.destructor.The result suggested that Dd-vap-1 may play an important role in the early stage when infecting host plants." @default.
- W2369291109 created "2016-06-24" @default.
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- W2369291109 date "2013-01-01" @default.
- W2369291109 modified "2023-09-27" @default.
- W2369291109 title "Cloning and Localization Analysis of a New Venom Allergen-like Protein Gene(Dd-vap-1) in Ditylenchus destructor" @default.
- W2369291109 hasPublicationYear "2013" @default.
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