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- W2369337158 abstract "Objective To study the feasibility of transplanting human saphanous vein endothelial cells to luminal surface of blood vessel prosthesis and to play a theoretical foundation for the clinical application of autologous endothelial cell transplantation. Methods Human saphanous vein endothelial cells were harvested with 0.1% collagenase and cultivated in vitro for 13.08± 1.24 days. The cultures were confirmed as endothelial cells with the fourescent linked anti Ⅷ antigen antibodies. The content of both 6 keto PGF1α and Von Willebrand factor (vWF) in the supernatant were detected with ELISA and radioimmunoassay. The multiplied cells were lined in vitro onto the luminal surface of expanded polytetraflouroethylene (ePTFE) grafts precoated with fibrin glue and fibronectin, then cultivated again for 9 days. Results 11.46±2.69×10 6 of available endothelial cells could be regularly obtained, the number of endothelial cells increased 147.93±88.68 times when culture were terminated. All the cells diploid cells with a purity of 99%. The content of both 6 keto PGF1α and vWF in the media showed no significant difference between the primary and subculture passages. The luminal surface of grafts was covered completely by a spindle like endothelial monolayer and an even fibrin glue matrix could be seen underneath. Conclusion Endothelial cells derived from human saphanous veins might be feasible to be transplanted onto the luminal surface of ePTFE and present a potential clinical application." @default.
- W2369337158 created "2016-06-24" @default.
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- W2369337158 date "2000-01-01" @default.
- W2369337158 modified "2023-09-25" @default.
- W2369337158 title "EXPERIMENTAL STUDIES OF TRANSPLANTATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN SA PHANOUS VEINS ONTO BLOOD VESSEL PROSTHESES" @default.
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