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- W2369393184 abstract "AIM: To observe the effect of different concentrations of glucose and homocysteine on the expression of MMP-9 in fibroblasts,and to investigate the relationship between homocysteine and diabetic foot. METHODS: Fibroblasts were obtained from the dermis of 1-day-old normal SD rat and cultured in DMEM containing 10% FBS. After deprivation of serum ( using 0. 5% FBS) for 24 h,the fibroblasts in 2-4 passages were cultured for 6 h under the conditions of normal glucose,high glucose and high glucose with high concentration of homocysteine,respectively. The expression of MMP-9 at mRNA and protein levels was assessed by RT-PCR and Western blotting,and the activity of MMP9 was determined by gelatin zymography analysis. RESULTS: The expression of MMP-9 at mRNA and protein levels and activity of MMP-9 in high glucose ( 22 mmol/L) group were 1. 15 folds,1. 59 folds and 1. 34 folds of those in normal glucose group ( P 0. 05) ,respectively. The effect of high glucose became more pronounced when co-treated with high concentration of homocysteine ( 100 μmol/L) ,which were 1. 25 folds,2. 63 folds and 2. 52 folds of those in normal glucose group ( P 0. 05) ,respectively. CONCLUSION: The increase in the expression of MMP-9 is concentration-dependen with glucose content in the culture. High concentration of homocysteine promotes this process,suggesting that hypercysteinemia might play an important role in the expression of MMP-9 in fibroblast." @default.
- W2369393184 created "2016-06-24" @default.
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- W2369393184 date "2010-01-01" @default.
- W2369393184 modified "2023-09-26" @default.
- W2369393184 title "Effects of high glucose and homocysteine on expression of MMP-9 in rat fibroblasts" @default.
- W2369393184 hasPublicationYear "2010" @default.
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