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- W2369395874 abstract "Objective To trans-differentiate from human amniotic epithelial cells to conjunctival epithelium under the specific condition.Methods Human amniotic epithelial cells were cultured under the inducing conditions of denuded conjunctival matrix and conjunctival homogenate.Immunohistochemistry with cytokeratin 4(CK4),CK13,MUC5AC,and PAS staining were performed respectively.The levels of MUC5AC in different time were measured by ELISA.The gene expressions of intracellular MUC5AC were tested by RT-PCR.Results After the induction of human amniotic epithelial cells,the expression of CK4,CK13 and MUC5AC in the test of immunohistochemistry was positive,and the positive cells of purplish red color were detected in PAS staining.Trace amount of MU5AC in culture medium was measured by ELISA and it increased gradually in day 1-7.The expression of MUC5AC in differentiated cells was positive shown by RT-PCR.Conclusion Human amniotic epithelial cells have a potential to differentiate to stem cells.In a specific condition,they can differentiate to conjunctival epithelium cells." @default.
- W2369395874 created "2016-06-24" @default.
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- W2369395874 date "2011-01-01" @default.
- W2369395874 modified "2023-09-27" @default.
- W2369395874 title "Experimental study on the differentiation from human amniotic epithelial cells to conjunctival epithelium cells" @default.
- W2369395874 hasPublicationYear "2011" @default.
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