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- W2369410435 abstract "t Objective:Our experiment discusses the effects of in vitro growth of the mouse embryonic fibroblasts (MEFs) that were treated by different concentrations of the homemade mytomycin for different times,and to provide the evidence for applications of homemade medicine in ES cells research.Method:To determine the growth curve of MEFs with the MTT method.Results:The homemade mytomycin(20 μg·mL~(-1) for 4 h,30 μg·mL~(-1) for 2 or 4 h) can inhibit effectively the division of the MEFs,but not affect vitality of the MEFs.Conclusion:The mytomycin produced by Zhejiang Hisun Pharmaceutical Co.,Ltd,not only can content the demand of preparation of feeder lay- ers,but also can reduce the experiment costs." @default.
- W2369410435 created "2016-06-24" @default.
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- W2369410435 date "2007-01-01" @default.
- W2369410435 modified "2023-09-25" @default.
- W2369410435 title "Application of domestic mytomycin on preparation of embryonic stem cells in its feeder layer" @default.
- W2369410435 hasPublicationYear "2007" @default.
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