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- W2369413179 abstract "【Objective】 In this study,a full-length cDNA for the anthocyanin synthase(ANS) gene from blueberry fruit was cloned,aiming to clarify the molecular mechanism of anthocyanin biosynthesis.【Method】 Using blueberry(Vaccinium spp.) cultivar 'Northland'(V.corymbosum L.) as material,a full-length cDNA sequence of ANS was obtained from skin of the blueberry using RT-PCR and RACE based on the differentially expressed unigene 38125 from the Illumina sequencing library(NCBI accession No.SRA046311).Using DNAMAN and MEGA software for multiple sequence alignment and phylogenetic analysis,the relationship between the relative expression of VcANS and anthocyanin content was investigated in different tissues and organs.【Result】 The cDNA sequence was named VcANS(GenBank accession No.JN654701).Sequence analyses indicated that VcANS was 1 424 bp in full length,containing a 5′-untranslated region(5′-UTR) of 93 bp,a 3′-UTR of 248 bp,and an opening reading frame(ORF) of 1 083 bp encoding a protein of 360 amino acids,which possessed the conserved structural domain of the 2-oxoglutarate and Fe2+-dependent oxygenase superfamily.Sequence alignment and phylogenetic tree analyses showed that VcANS had the closest phylogenetic relationship with the Ericaceae.The relative expression of VcANS had significant consistency with the content of anthocyanins.【Conclusion】 A full-length anthocyanin synthase gene was obtained by using RT-PCR and RACE techniques,which will lay the molecular biology foundation for further research on the molecular mechanism of anthocyanin biosynthesis and new varieties breeding of blueberry." @default.
- W2369413179 created "2016-06-24" @default.
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- W2369413179 date "2012-01-01" @default.
- W2369413179 modified "2023-09-25" @default.
- W2369413179 title "Molecular cloning and expression analysis of VcANSgene in blueberry" @default.
- W2369413179 hasPublicationYear "2012" @default.
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