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- W2369448801 abstract "Citrus tatter leaf virus(CTLV) is an economically important pathogen of citrus.To establish a sensitive and specific method for detection of this virus,a nested RT-PCR was developed by comparing the detection efficiency of five primer sets.Total nucleotide acid extraction of CTLV-inoculated samples were serially tenfold diluted to evaluate the sensitivity,and samples infected with CTV,SDV,HLB,Canker,or CEVd were used to determine the specificity.The results showed that the lowest detection limit of the total nucleic acid by the method reached about 1.27 μg·L-1,which was hundred times lower than that of One-step RT-PCR assay at least.No positive result was obtained from the samples without CTLV using this method.In the detection of 142 samples collected from orchards,24 shoot-tip grafting citrus,and 20 rusk citrange infected by CTLV,the positive rates using the nested RT-PCR,the routine semi-nested PCR,and the one-step RT-PCR were 21.5%,18.3% and 19.4% respectively.It suggested that the nested RT-PCR is a sensitive,specific method and can be used as a useful tool for CTLV detection." @default.
- W2369448801 created "2016-06-24" @default.
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- W2369448801 date "2011-01-01" @default.
- W2369448801 modified "2023-10-06" @default.
- W2369448801 title "Establishment of nested RT-PCR for detecting Citrus tatter leaf virus" @default.
- W2369448801 hasPublicationYear "2011" @default.
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