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- W2369456806 abstract "OBJECTIVE:To develop a quantitative method for IL 1β mRNA expression, and use it for drug screening. METHODS: Το use house keeping gene G3PDH as an internal standard to quantitate the mRNA expression with one step RT PCR. RESULTS: We developed the method of quantitative RT PCR, and confirmed the method by ELISA and slot blot with dexamethasone as positive control.CONCLUSION: The method quantitating IL 1β mRNA expression is efficient, sensitive and reliable." @default.
- W2369456806 created "2016-06-24" @default.
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- W2369456806 date "2001-01-01" @default.
- W2369456806 modified "2023-09-24" @default.
- W2369456806 title "THE DEVELOPMENT AND APPLICATION OF ONE STEP RT PCR, A METHOD OF DRUG SCREENING,FOR MEASURING HUMAN IL 1β mRNA EXPRESSION" @default.
- W2369456806 hasPublicationYear "2001" @default.
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