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- W2369557647 abstract "The experiment was conducted to study cloning、sequence、 expression and functional identification of thermophilic neutral protase(tnp) gene in this paper.The Bacillus licheniformis XJT9503 gene DNA was produced as a template by thermophilic neutral potase.The fragment encoding tnp was obtained by amplifying PCR,which showed and open reading frame through sequence analysis.The plasmid pPL-tnp was obtained by B. subtilis vector pPL608.Then the plasmid pPL-tnp was introduced into the protease-deficient mutant B.subtilis AB97013 to secrete and express,the enzyme characteristics for the tnp was the same as Bacillus licheniformi about the optimum reaction conditons of temperature and pH,which suggested the tnp gene was abtained,cloned and expressed accurately." @default.
- W2369557647 created "2016-06-24" @default.
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- W2369557647 date "2008-01-01" @default.
- W2369557647 modified "2023-09-26" @default.
- W2369557647 title "Study on the Cloning、Expression and Functional Identification of Thermophilic Neutral Protase Gene" @default.
- W2369557647 hasPublicationYear "2008" @default.
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