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- W2369584396 abstract "Objective:To determine whether it is possible to enhance human periodontal ligament fibroblasts attachment and proliferation to human diseased root surface treated with EDTA and Platelet-poor plasma(PPP) . Methods:There were four groups involved in the experiment:the untreated root slices,the root slices treated with EDTA,the root slices treated with PPP alone and treated with EDTA and PPP. We fixed the root slices to the bottom of wells in a 48-well tissue culture plate. All test slices were then sterilized under UV light for 2 hours prior to seeding with Cells. The culture was cultured for 24 or 48 hours in standard incubation conditions. The cell attachment was assessed by cell counting and the proliferation was assessed by using MTT assay. Cell morphology and growth pattern were observed by scanning electron microscopy. Results:There were the least number of attached cells on the untreated root surfaces. Treatment of the root surfaces with EDTA or PPP alone significantly increased cell attachment for HPDLF(P0.05) . When PPP was added to previously EDTA-etched root surfaces,cell attachment was further enhanced(P0.01) . There was no cell proliferation on the untreated diseased root surfaces in 48h comparing to 24h(P0.05) . The results showed that there was slightly increased cell proliferation on the diseased root surfaces treated with EDTA or PPP alone,but there was no statistical significance. When PPP was added to previously EDTA-etched root surfaces,cell proliferation was obviously enhanced(P0.05) . Conclusion:Pretreatment of diseased human root surfaces with freshly prepared PPP or EDTA may enhance the attachment and proliferation of human periodontal ligament fibroblasts." @default.
- W2369584396 created "2016-06-24" @default.
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- W2369584396 date "2010-01-01" @default.
- W2369584396 modified "2023-09-22" @default.
- W2369584396 title "Attachment and Proliferation of Human Periodontal Ligament Fibroblasts to Diseased Root Surfaces Affected by Platelet-Poor Plasma and Demineralization in Vitro" @default.
- W2369584396 hasPublicationYear "2010" @default.
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