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- W2369654074 abstract "Objective:To construct the recombinant plasmid of HPV16L1-E7C and study its cel-lular immunity.Methods:The HPV16L1-E7C DNA fragment was inserted into the pcDNA3.1vector by gene cloning.Then the plasmid pcDNAL1-E7C was used to immunize female C57BL/6mice three times at2weeks intervals.T lymphocyte proliferation test and ELISA were used to detect the specific cellular immunity of recombinant plasmid.Result:In the T cell proliferation test,the 3 H-TdR incorpo-ration in the experimental group was18339±1972and6737±1243in the control group,showing a higher in the experimental group than in the control group(P0.05).After DNA immunization,the se-cretion level of IFN-γin the experimental group was0.89±0.56and that in the control group was4.81±1.33,with a statislically significant difference(P0.05).Conclusion:The recombinant plasmid could significantly enhance the cellular immunity of mice." @default.
- W2369654074 created "2016-06-24" @default.
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- W2369654074 date "2003-01-01" @default.
- W2369654074 modified "2023-09-28" @default.
- W2369654074 title "Construction of recombinant plasmid of HPV16L1-E7C and the primary study of its cellular immunity" @default.
- W2369654074 hasPublicationYear "2003" @default.
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