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- W2369659800 abstract "The full length cDNA of arginine kinase gene(HdAK)from Haliotis diversicolor was cloned for the first time by SMART-RACE method.The mRNA expression levels of HdAK in examined tissues and in gill and haemocytes under thermal stress,hypoxia exposure,thermal plus hypoxia stress and the injection of Vibrio parahaemolyticus were analyzed by real-time quantitative PCR(qRT-PCR).Results showed that the full length cDNA sequence of HdAKis 1 595 bp and encodes 354 amino acids.The qRT-PCR results showed that HdAK gene was detected in all examined tissues and with the highest expression level in hepatopancreas(p 0.05).Under different stresses,the mRNA expression levels in either gills or haemocytes of HdAK gene were signicantly up-regulated in some phases.These results indicated that HdAK is involved in response to energy metabolism and plays roles in response to thermal,hypoxia stress and the injection of V.parahaemolyticus.These results will facilitate the study of the physiological mechanisms of these stresses on H.diversicolor." @default.
- W2369659800 created "2016-06-24" @default.
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- W2369659800 date "2015-01-01" @default.
- W2369659800 modified "2023-09-25" @default.
- W2369659800 title "Cloning and Differential Expression of Arginine Kinase Gene in Haliotis diversicolor under Different Stresses" @default.
- W2369659800 hasPublicationYear "2015" @default.
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