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- W2369694899 abstract "Hantavirus,the causative agent of human hemorrhagic fever with renal syndrome(HFRS),possesses a tripartite,single-stranded RNA genome,with segments designated as small(S),medium(M)and large(L)encoding the viral nucleocapsid protein,envelope glycoproteins(G1 and G2)and polymerase protein,respectively.A unique structural feature of the viral genome is having about 18-nucleotide highly conserved inverted repeat sequences presenting at the terminals of each gene segment in different types of Hantavirus.This inverted repeat sequence at the ends of each gene segment can form a special hairpin structure when it got complemented.However,the function of the hairpin structure has not been clearly known.In this study,we expressed in E.coli the nucleocapsid proteins(NP)of two Chinese Hantavirus vaccine strains Z10(Hantaan type)and Z37(Seoul type),isolated from a HFRS patient and Rattus norvegicus, respectively.Using a UNICORE~ (TM) FPLC system,the His-tagged recombinant nucleocapsid proteins were then purified with Ni-NAT affinity chromatography and DEAE ion exchange chromatography.The purified recombinant NPs were then examined and distinguished by trypsin incomplete digestion,followed by Western blotting using anti-His monoclonal antibody.Electrophoresis mobility shift assay(EMSA)showed that both NPs of Z10 and Z37 hantaviruses bound specifically to the double-stranded DNA probe possessing the 18-nucleotide inverted repeat sequences in vitro. These data suggest that the inverted repeat sequence at the ends of each gene segment could be an important target site for nucleocapsid protein,and therefore plays an important role during Hantavirus packaging to assemble the viral apparatus into a complete viral particle." @default.
- W2369694899 created "2016-06-24" @default.
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- W2369694899 date "2004-01-01" @default.
- W2369694899 modified "2023-09-25" @default.
- W2369694899 title "Expression, purification and characterization of nucleocapsid protein of hantavirus Chinese vaccine strains Z10 and Z37: nucleocapsid protein specifically binds to the inverted repeat sequences conserved at terminals of hantavirus genome {sl in vitro}" @default.
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