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- W2369700597 abstract "【Objective】This research aimed to clone the full length cDNA of chitinase gene fromPopulus szechuanica and analyze its expression characteristics during pathogenesis process.【Method】The cDNA of chitinase gene(GenBank accession:KC416180)fromP.szechuanicaleaves infected by Melampsora larici-populina Sb052was cloned using RACE technology,and analyzed by bioinformatics method and real-time quantitative PCR.【Result】Full length of PsChiⅠ gene(GenBank accession number:KC416180)with 1 145bp in length was obtained.It contained a 38bp 5′-untanslated region(5′-UTR),a 147bp 3′-UTR,and a 960bp open reading frame(ORF).The predicted protein encoded 319amino acid residues and contained two sequences belonging to the 19th family signature sequences of chitinases.Its theoretical isoelectric point was 5.03.The gene obtained from P.szechuanica belonged to ClassⅠb acidic chitinase,a member of the 19th chitinase family.Sequence alignment and phylogenetic tree revealed that the deduced amino acid sequence of PsChiⅠhad the highest similarity and closest phylogenetic relationship with P.trichocarpa.QRT-PCR analysis showed that PsChiⅠ gene expressed in all pathogenesis processes and the highest relative expression levels occurred at 12hpi and 48hpi.【Conclusion】A full length of chitinase gene was obtained,and its role in poplar against fungal infection was analyzed." @default.
- W2369700597 created "2016-06-24" @default.
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- W2369700597 date "2014-01-01" @default.
- W2369700597 modified "2023-09-25" @default.
- W2369700597 title "Cloning and expression of PsChiIgene from poplar" @default.
- W2369700597 hasPublicationYear "2014" @default.
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